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KMID : 0387420060170020015
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Korean Society of Oriental Neuropsychiatry
2006 Volume.17 No. 2 p.15 ~ p.36
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The effects of Chenwangbosim-Dan and herbs on Mouse neuroblastoma 2a cells damaged by hypoxia-reoxygenation
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Kim Sang-Ho
Kim Jong-Woo
Kang Chul-Hun
Hwang Wei-Wan
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Abstract
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Objective:This study was designed to assess effect of and Chenwangbosim-Dan(CWBSD) herbs on Mouse neuroblastoma 2a cells damaged by hypoxia-reoxygenation.
Method:Mouse neuroblastoma 2a (N2a) cells were measured by MTT assay and LDH assay after 48h hypoxia and 6h reoxygenation. Mouse neuroblastoma 2a (N2a) cells were treated by CWBSD and herbs.
Result:1. In MTT assay of hypoxia CWBSD and BJI, SJH, IS, CHR, HS among all of herbs were effective. Especially CWBSD and IS were highly effective. 2. In MTT assay of reoxygenation SJI, SJH, VJ, IS, BJI were effective. Especially SJI, SJH, YJ were highly effective. 3. In LDH assay of hypoxia CWBSD, DS, DG, SJH, OMZ were effective. Especially CWBSD, DG were highly effective. 4. In LDH assay of reoxygenation all of herbs except CWBSD and BJI were generally effective. Especially CHR, SJH, YJ, OMZ, HS were highly effective.
Conclusion:The results suggest that CWBSD, and it’s ingradient(especially SJH, CHR and SJI) may have protective effect on condition of oxidative stress.
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KEYWORD
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Chenwangbosim-Dan, oxidative stress, hypoxia-reoxygenation
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